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Event: 1560
Key Event Title
Altered differentiation
Short name
Biological Context
Level of Biological Organization |
---|
Cellular |
Cell term
Organ term
Key Event Components
Key Event Overview
AOPs Including This Key Event
AOP Name | Role of event in AOP | Point of Contact | Author Status | OECD Status |
---|---|---|---|---|
HDAC inhibition leads to neural tube defects | KeyEvent | Allie Always (send email) | Under Development: Contributions and Comments Welcome |
Taxonomic Applicability
Term | Scientific Term | Evidence | Link |
---|---|---|---|
Mus musculus | Mus musculus | Moderate | NCBI |
Life Stages
Life stage | Evidence |
---|---|
Birth to < 1 month | |
Pregnancy | Moderate |
Sex Applicability
Term | Evidence |
---|---|
Unspecific | Moderate |
Key Event Description
Proper differentiation during embryonic development is regulated by the expression of genes at the right time and space. If key regulator genes are not expressed or wrongly expressed this leads to a different cell type.
How It Is Measured or Detected
Differentiation can be measured e.g. by in vitro hESC or iPSC based differentiation systems. Pre-requisite for this is a well characterized and homogenous cell population. Then it can be measured by the analysis of altered genes by gene set enrichment analysis (GEA) comparing control with potentially disturbed differentiation.
In the context of embryonic brain development, immunofluorescence on brain sections can be used with antibodies against neuronal differentiation markers such Tuj1, NeuN and betaIII-tubulin.
Domain of Applicability
Embryonic development