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Key Event Title
Decreased proximal tubular vectorial transport
|Level of Biological Organization|
Key Event Components
Key Event Overview
AOPs Including This Key Event
|AOP Name||Role of event in AOP||Point of Contact||Author Status||OECD Status|
|Complex I inhibition leads to Fanconi syndrome||KeyEvent||Evgeniia Kazymova (send email)||Under development: Not open for comment. Do not cite|
Key Event Description
Vectorial transport across the monolayer is the primary function of proximal tubule cells in the nephron. The cells transport both water and solutes via active and secondary active transporters.
How It Is Measured or Detected
When proximal tubule cells are grown on filter inserts, the volume and composition of the medium in the apical and basolateral compartments can be studied separately to determine variations in a vectorial transport.
Fluorescent dyes can also be used to measure transport across the monolayer either directly (transport of the dye itself) or indirectly (competition with the dye).
A differential measure of the rate of water transport can also be used in this format (Wilmes et al. 2014)
Domain of Applicability
Wilmes, A., L. Aschauer, A. Limonciel, W. Pfaller, and P. Jennings. 2014. “Evidence for a Role of Claudin 2 as a Proximal Tubular Stress Responsive Paracellular Water Channel.” Toxicology and Applied Pharmacology 279(2)