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Key Event Title
Reduction, 17-OH-pregnenolone conversion in DHEA
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Key Event Components
Key Event Overview
AOPs Including This Key Event
|AOP Name||Role of event in AOP||Point of Contact||Author Status||OECD Status|
|Cyp17A1 inhibition leads to undescended testes in mammals||KeyEvent||Evgeniia Kazymova (send email)||Open for citation & comment|
Key Event Description
17-OH-pregnenolone is the direct precursor of dehydroepiandrosterone (DHEA), a reduction in its synthesis results in a decrease in DHEA level. DHEA is defined as an obligatory intermediate in sex steroid biosynthesis being the precursor of steroid hormones like testosterone and estradiol.12
How It Is Measured or Detected
17-OH-pregnenolone and DHEA can be fractionated using High Performance Liquid Chromatography. After separation, pregnenolone and DHEA levels can be quantify using immunoassay such as ELISA or Radio Immuno Assay (RIA). For both steroids, LC-MS/MS is also an option.
Domain of Applicability
1 Miller Walter L. (1988) Molecular Biology of Steroid Hormone Synthesis. Endocrine Reviews, 9(3): 295-318. https://doi.org/10.1210/edrv-9-3-295
2 Miller W.L. and Auchus R.J. (2011) The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders. Endocrine Reviews, 32(1): 81-151.https://doi.org/10.1210/er.2010-0013