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Key Event Title
Increase, Lipid peroxidation
|Level of Biological Organization|
Key Event Components
Key Event Overview
AOPs Including This Key Event
|AOP Name||Role of event in AOP||Point of Contact||Author Status||OECD Status|
|Excessive ROS leading to mortality (3)||KeyEvent||Arthur Author (send email)||Under development: Not open for comment. Do not cite|
|Uncoupling of OXPHOS leading to growth inhibition 5||KeyEvent||Arthur Author (send email)||Under development: Not open for comment. Do not cite||Under Development|
|Oxidation of Reduced Glutathione Leading to Mortality||KeyEvent||Agnes Aggy (send email)||Open for citation & comment|
Key Event Description
Lipid peroxidation is the direct damage to lipids in the membrane of the cell or the membranes of the organelles inside the cells. Ultimately the membranes will break due to the build-up damage in the lipids This is mainly caused by oxidants which attack lipids specifically, since these contain carbon-carbon double bonds. During lipid peroxidation several lipid radicals are formed in a chain reaction. These reactions can interfere and stimulate each other. Antioxidants, such as vitamin E, can react with lipid peroxy radicals to prevent further damage in the cell (Cooley et al. 2000).
How It Is Measured or Detected
The main product of lipid peroxidation, malondialdehyde is used to measure the degree of this process occurring. Malondialdehyde is primarily measured via an aldehyde product of its fluorometric and colorimetric assays. The thiobarbituric acid reactive substances assay is a very common assay that is used to detect and quantify lipid peroxidation that is both colorimetric and fluorimetric. However, it is most often practiced as fluorometric/spectrophotmetric. Other methods include measuring other aldehyde products of lipid peroxidase such as 4-hydroxyalkenals (alongside malondialdehyde) using similar assays.
Domain of Applicability
ROS is a normal constituent found in all organisms, therefore, all organisms containing lipid membranes may be affected by lipid peroxidation.
Structure: Regardless of sex or life stage, when exposed to free radicals, there is potential for lipid peroxidation as a auxiliary response where there are lipid membranes.
Cooley HM, Evans RE, Klaverkamp JF. 2000. Toxicology of dietary uranium in lake whitefish (Coregonus clupeaformis). Aquatic Toxicology. 48(4):495–515. https://doi.org/10.1016/S0166-445X(99)00057-0