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Event: 1686

Key Event Title

A descriptive phrase which defines a discrete biological change that can be measured. More help

Deposition of Energy

Short name

The KE short name should be a reasonable abbreviation of the KE title and is used in labelling this object throughout the AOP-Wiki. More help

Energy Deposition

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Biological Context

Structured terms, selected from a drop-down menu, are used to identify the level of biological organization for each KE. More help

Level of Biological Organization
Molecular

Cell term

The location/biological environment in which the event takes place.The biological context describes the location/biological environment in which the event takes place. For molecular/cellular events this would include the cellular context (if known), organ context, and species/life stage/sex for which the event is relevant. For tissue/organ events cellular context is not applicable. For individual/population events, the organ context is not applicable. Further information on Event Components and Biological Context may be viewed on the attached pdf. More help

Organ term

Key Event Components

The KE, as defined by a set structured ontology terms consisting of a biological process, object, and action with each term originating from one of 14 biological ontologies (Ives, et al., 2017; https://aopwiki.org/info_pages/2/info_linked_pages/7#List). Biological process describes dynamics of the underlying biological system (e.g., receptor signalling).Biological process describes dynamics of the underlying biological system (e.g., receptor signaling). The biological object is the subject of the perturbation (e.g., a specific biological receptor that is activated or inhibited). Action represents the direction of perturbation of this system (generally increased or decreased; e.g., ‘decreased’ in the case of a receptor that is inhibited to indicate a decrease in the signaling by that receptor). Note that when editing Event Components, clicking an existing Event Component from the Suggestions menu will autopopulate these fields, along with their source ID and description. To clear any fields before submitting the event component, use the 'Clear process,' 'Clear object,' or 'Clear action' buttons. If a desired term does not exist, a new term request may be made via Term Requests. Event components may not be edited; to edit an event component, remove the existing event component and create a new one using the terms that you wish to add. Further information on Event Components and Biological Context may be viewed on the attached pdf. More help

Key Event Overview

AOPs Including This Key Event

All of the AOPs that are linked to this KE will automatically be listed in this subsection. This table can be particularly useful for derivation of AOP networks including the KE. Clicking on the name of the AOP will bring you to the individual page for that AOP. More help

AOP Name	Role of event in AOP	Point of Contact	Author Status	OECD Status
Deposition of energy leading to lung cancer	MolecularInitiatingEvent	Brendan Ferreri-Hanberry (send email)	Under development: Not open for comment. Do not cite	EAGMST Approved
Ionizing Radiation-Induced AML	MolecularInitiatingEvent	Allie Always (send email)	Under development: Not open for comment. Do not cite
ROS production leading to population decline via photosynthesis inhibition	MolecularInitiatingEvent	Arthur Author (send email)	Under development: Not open for comment. Do not cite
ROS production leading to population decline via mitochondrial dysfunction	MolecularInitiatingEvent	Agnes Aggy (send email)	Under development: Not open for comment. Do not cite
DNA damage leading to population decline via programmed cell death	MolecularInitiatingEvent	Allie Always (send email)	Under development: Not open for comment. Do not cite
Deposition of ionising energy leads to population decline via pollen abnormal	MolecularInitiatingEvent	Brendan Ferreri-Hanberry (send email)	Under development: Not open for comment. Do not cite
Deposition of energy leading to population decline via DSB and follicular atresia	MolecularInitiatingEvent	Evgeniia Kazymova (send email)	Under development: Not open for comment. Do not cite
Deposition of energy leading to population decline via DSB and apoptosis	MolecularInitiatingEvent	Agnes Aggy (send email)	Under development: Not open for comment. Do not cite
Energy deposition leading to population decline via DNA oxidation and oocyte apoptosis	MolecularInitiatingEvent	Agnes Aggy (send email)	Under development: Not open for comment. Do not cite
Energy deposition leading to population decline via DNA oxidation and follicular atresia	MolecularInitiatingEvent	Allie Always (send email)	Under development: Not open for comment. Do not cite
Increased DNA damages during embryonic development lead to microcephaly	MolecularInitiatingEvent	Brendan Ferreri-Hanberry (send email)	Under development: Not open for comment. Do not cite
Deposition of energy leads to reduced cocoon hatchability	MolecularInitiatingEvent	Allie Always (send email)	Under development: Not open for comment. Do not cite

Taxonomic Applicability

Latin or common names of a species or broader taxonomic grouping (e.g., class, order, family) that help to define the biological applicability domain of the KE.In many cases, individual species identified in these structured fields will be those for which the strongest evidence used in constructing the AOP was available in relation to this KE. More help

Term	Scientific Term	Evidence	Link
human	Homo sapiens	High	NCBI
rat	Rattus norvegicus	High	NCBI
mouse	Mus musculus	High	NCBI

Life Stages

An indication of the the relevant life stage(s) for this KE. More help

Life stage	Evidence
All life stages	High

Sex Applicability

An indication of the the relevant sex for this KE. More help

Term	Evidence
Unspecific	High

Key Event Description

A description of the biological state being observed or measured, the biological compartment in which it is measured, and its general role in the biology should be provided. More help

Deposition of energy refers to events where subatomic particles or electromagnetic waves of sufficient energy cause ionization in the media through which they transverse (Beir, 1999). The resulting energy can cause the ejection of electrons from atoms and molecules, thereby breaking chemical bonds and ionizing atoms and molecules. The energy of these subatomic particles or electromagnetic waves ranges from 124 KeV to 5.4 MeV, and is dependent on the source and type of radiation. Not all electromagnetic radiation is ionizing; as the incident radiation must have sufficient energy to free electrons from the atom or molecule’s electron orbitals. The energy can induce direct and indirect ionization events. Direct ionization is the principal path where charged particles interact with DNA to cause a biological damage. Photons, which are electromagenetic waves can also cause direct ionization. Indirect ionization produces free radicals of other molecules, specifically water, which can transform to damage critical targets such as DNA (Beir, 1999). There are no chemical mimetics or prototypes of energy deposition. Given the fundamental nature of energy deposition by nuclei, nucleons or elementary particles in material, this process is universal to all biological contexts. It is a phenomenon dictated by radioactive decay laws. As such chemical initiators are also not applicable to this MIE.

How It Is Measured or Detected

A description of the type(s) of measurements that can be employed to evaluate the KE and the relative level of scientific confidence in those measurements.These can range from citation of specific validated test guidelines, citation of specific methods published in the peer reviewed literature, or outlines of a general protocol or approach (e.g., a protein may be measured by ELISA). Do not provide detailed protocols. More help

Assay Name	References	Description	OECD Approved Assay
Monte Carlo Simulations (Geant4)	Douglass et al., 2013; Douglass et al. 2012	Monte Carlo simulations are based on a computational algorithm that mathematically models the deposition of energy into materials.	N/A
Fluorescent Nuclear Track Detector (FNTD)	Sawakuchi, 2016; Niklas, 2013; Koaira et al., 2015	FNTDs are biocompatible chips with crystals of aluminium oxide doped with carbon and magnesium; used in conjuction with fluorescent microscopy, these FNTDs allow for the visualization and the linear energy transfer (LET) quantification of tracks produced by the deposition of energy into a material.	N/A

Domain of Applicability

A description of the scientific basis for the indicated domains of applicability and the WoE calls (if provided). More help

Energy can be deposited into any substrate, both living and non-living; it is independent of age, taxa, sex, or life-stage.

References

List of the literature that was cited for this KE description. More help

Alloni, AD. et al.(2014),” Modeling Dose Deposition and DNA Damage Due to Low-Energy β – Emitters.”, Radiation Research.182(3):322–330. doi:10.1667/RR13664.1.

Beir, V. et al. (1999), “ The Mechanistic Basis of Radon-Induced Lung Cancer.”, https://www.ncbi.nlm.nih.gov/books/NBK233261/.

Douglass, M. et al. (2013),” Monte Carlo investigation of the increased radiation deposition due to gold nanoparticles using kilovoltage and megavoltage photons in a 3D randomized cell model.”, Med Phys. 40(7), 071710. doi:10.1118/1.4808150.

Douglass, M. et al. (2012),” Development of a randomized 3D cell model for Monte Carlo microdosimetry simulations.”, Med Phys. 39(6):3509-3519, doi:10.1118/1.4719963.

Friedland, W. et al. (2017),” Comprehensive track-structure based evaluation of DNA damage by light ions from radiotherapy- relevant energies down to stopping.”, Nat Publ Gr.1–15. doi:10.1038/srep45161.

Hada, M. & Georgakilas, AG. (2008), “Formation of Clustered DNA Damage after High-LET Irradiation.” J Radiat Res. 49(3):203–210. doi:10.1269/jrr.07123.

Hunter, N. & Muirhead, CR. (2009).” Review of relative biological effectiveness dependence on linear energy transfer for low-LET radiations Review of relative biological effectiveness dependence.”, Journal of Radiological Protection. 29(1):5-21. doi:10.1088/0952-4746/29/1/R01.

Kodaira, S. & Konishi, T. (2015), “Co-visualization of DNA damage and ion traversals in live mammalian cells using a fluorescent nuclear track detector.” Journal of Radiation Research. 360–365. doi:10.1093/jrr/rru091.

Liamsuwan, T. (2014).” Microdosimetry of proton and carbon ions.”, Med Phys. 41(8):081721. doi: 10.1118/1.4888338.

Lorat, Y. (2015),” Nanoscale analysis of clustered DNA damage after high-LET irradiation by quantitative electron microscopy – The heavy burden to repair.”, DNA Repair (Amst). 28:93–106. doi:10.1016/j.dnarep.2015.01.007.

Nikitaki, Z. et al. (2016), “Measurement of complex DNA damage induction and repair in human cellular systems after exposure to ionizing radiations of varying linear energy transfer ( LET ).”,Free Radical Research. 50(sup1):S64-S78.doi:10.1080/10715762.2016.1232484.

Niklas, M. et al. (2013), “Engineering cell-fluorescent ion track hybrid detectors.”, Radiation Oncology. 8:141. doi: 10.1186/1748-717X-8-141.

Okayasu, R. (2012a), “heavy ions — a mini review.”, Int J Cancer. 1000:991–1000. doi:10.1002/ijc.26445.

Okayasu, R. (2012b), “Repair of DNA damage induced by accelerated heavy ions-A mini review.”, Int J Cancer. 130(5):991–1000. doi:10.1002/ijc.26445.

Sawakuchi, GO. & Akselrod, MS. (2016), “Nanoscale measurements of proton tracks using fluorescent nuclear track detectors.”,Med Phys. 43(5):2485–2490. doi:10.1118/1.4947128.