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Event: 1771
Key Event Title
Decrease, Adenosine triphosphate pool
Short name
Biological Context
Level of Biological Organization |
---|
Cellular |
Cell term
Cell term |
---|
cell |
Organ term
Key Event Components
Process | Object | Action |
---|---|---|
ATP biosynthetic process | ATP | decreased |
Key Event Overview
AOPs Including This Key Event
AOP Name | Role of event in AOP | Point of Contact | Author Status | OECD Status |
---|---|---|---|---|
Excessive ROS leading to mortality (2) | KeyEvent | Brendan Ferreri-Hanberry (send email) | Under development: Not open for comment. Do not cite | |
Excessive ROS leading to mortality (3) | KeyEvent | Arthur Author (send email) | Under development: Not open for comment. Do not cite | |
Uncoupling of OXPHOS leading to growth inhibition 2 | KeyEvent | Evgeniia Kazymova (send email) | Open for citation & comment | Under Development |
Uncoupling of OXPHOS leading to growth inhibition 1 | KeyEvent | Allie Always (send email) | Open for citation & comment | WPHA/WNT Endorsed |
Thermal stress leading to population decline (1) | KeyEvent | Agnes Aggy (send email) | Under development: Not open for comment. Do not cite | |
Mitochondrial ATP synthase antagonism leading to growth inhibition (1) | KeyEvent | Brendan Ferreri-Hanberry (send email) | Under development: Not open for comment. Do not cite | |
Mitochondrial ATP synthase antagonism leading to growth inhibition (2) | KeyEvent | Arthur Author (send email) | Under development: Not open for comment. Do not cite | |
Mitochondrial complex III antagonism leading to growth inhibition (1) | KeyEvent | Agnes Aggy (send email) | Under development: Not open for comment. Do not cite | |
Mitochondrial complex III antagonism leading to growth inhibition (2) | KeyEvent | Allie Always (send email) | Under development: Not open for comment. Do not cite | |
Uncoupling of OXPHOS leading to growth inhibition 4 | KeyEvent | Brendan Ferreri-Hanberry (send email) | Under development: Not open for comment. Do not cite | Under Development |
Taxonomic Applicability
Life Stages
Life stage | Evidence |
---|---|
Embryo | High |
Juvenile | High |
Adult, reproductively mature | Moderate |
Sex Applicability
Term | Evidence |
---|---|
Unspecific | High |
Key Event Description
Decreased adenosine triphosphate (ATP) pool describes the loss of balance between ATP synthesis and ATP consumption, leading to reduced total ATP. As a primary form of biological energy, ATP is used by many biological processes (Bonora 2012). Decrease in ATP level normally attributes to metabolic disorders in major ATP synthetic pathways, such as mitochondrial oxidative phosphorylation, fatty acid β-oxidation, glycolysis and plant photophosphorylation.
How It Is Measured or Detected
-The ATP pool in cells or tissue can be quantified using a well-established ATP bioluminescent assay (Lemasters 1978; Wibom 1990). Assay principles: ATP can react with luciferase and luciferin from firefly and the luminescence emitted from the reaction is proportional to the ATP concentration:
ATP + D-Luciferin + O2 è Oxyluciferin + AMP + PPi + CO2 + Light
-ToxCast high-throughput screening bioassays, such as “NCCT_HEK293T_CellTiterGLO” and “NIS_HEK293T_CTG_Cytotoxicity” can be used to measure this KE.
Domain of Applicability
Taxonomic applicability domain
This key event is in general considered applicable to all eukaryotes utilizing ATP as a direct source of energy and signaling molecule.
Life stage applicability domain
This key event is considered applicable to all life stages, as all developmental stages require energy supply to maintain necessary physiological processes.
Sex applicability domain
This key event is considered sex-unspecific, as both males and females use ATP as an essential energy molecule.
References
Bonora M, Patergnani S, Rimessi A, De Marchi E, Suski JM, Bononi A, Giorgi C, Marchi S, Missiroli S, Poletti F, Wieckowski MR, Pinton P. 2012. ATP synthesis and storage. Purinergic Signalling 8:343-357. DOI: 10.1007/s11302-012-9305-8.
Lemasters JJ, Hackenbrock CR. 1978. [4] Firefly luciferase assay for ATP production by mitochondria. Methods in Enzymology. Vol 57. Academic Press, pp 36-50.
Wibom R, Lundin A, Hultman E. 1990. A sensitive method for measuring ATP-formation in rat muscle mitochondria. Scandinavian Journal of Clinical and Laboratory Investigation 50:143-152. DOI: 10.1080/00365519009089146.